Abstract:

Preparation, regeneration, UV mutagenization of Ganoderma lucidum protoplast were studied to obtain high-yield strains producing organogemanium. Under optimized conditions for the preparation and regeneration of Ganoderma lucidum protoplast including cell age 96 h, lywallzyme concentration 2%, glusulase concentration 1%, cellulase concentration 1%, and temperature 31 ℃for an enzymolysis duration of 2 h, the protoplast yield and regeneration rate were found to be 4.33 × 106 protoplasts/ml and 0.00275%, respectively. When Ganoderma lucidum protoplast was irradiated for 30 s with 15 W ultraviolet lamp 30 cm away from it, the lethality was 66.7% and three high-yield strains were obtained after two passages, the organogemanium contents in whose dried mycelia were higher than that of starting strain by 113.58%, 105.87% and 103.86% separately.

Key words: UV mutagenization, protoplast, Ganoderma lucidum, organogemanium